The Zygophyllaceae family has about 27 genera and 350 species worldwide, mainly in Asia, Africa, Europe, and Australia. The plants of this family are rich in resources and are valuable for in-depth research.
Some plants in the Zygophyllaceae family are mainly propagated by seeds, which have low germination rates and irregular germination, and seed propagation is prone to interspecific variation and intergenerational degeneration. Lifeasible used tissue culture rapid propagation technology to obtain high-quality, neat Zygophyllaceae seedlings, and the use of our system allows for the rational exploitation and sustainable use of Zygophyllaceae plant resources.
Protoplast culture is the basis of genetic engineering, so we have also established a protoplast culture system isolated from healing tissues, and tissue culture of some Zygophyllaceae plants with leaves, stem segments, and hypocotyls of sterile seedlings as explants, and a high-frequency regeneration system.
Figure 1. Protoplast culture system isolated from healing tissues.
Lifeasible used Zygophyllaceae plant healing tissues as the material for isolating protoplasts, because the healing tissues are easy to obtain, not limited by time and season, and can be supplied year-round, thus also ensuring the material supply for the high-frequency regeneration system we established.
Plant protoplasts can be induced to fuse and bypass the sexual incompatibility barrier because there is no barrier to the cell wall. Therefore, our established regeneration system for Zygophyllaceae plants can be used for distant interspecific somatic hybridization and as an ideal recipient for genetic engineering studies.
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