Taxaceae Plant Tissue Culture

Leaves and mature aril of Taxus chinensis belonging to
the Taxaceae.
Our Taxaceae plant tissue culture technology service
can be used for the rapid propagation of Taxus
chinensis for the mass extraction of paclitaxel with
anticancer activity.

Taxaceae belongs to the order Pinaceae, which includes 7 genera and about 30 species of plants distributed in North America, North Africa, and Eurasia to Southeast Asia. Taxaceae plants are mostly evergreen, branching small trees and shrubs. The leaves are spirally arranged and linear to lance-shaped. They are dioecious and rarely monoecious. Seeds are nut-like, enclosed in an aril. The mature aril is brightly colored and soft, but the seeds contain the highly toxic substances paclitaxel.

Taxaceae plants are slow-growing, solid, hard, tough, meticulously structured, uniformly textured, and resistant to decay, making them high-quality wood for fine furniture and carvings. Most species have dark green leaves throughout the year, and in autumn, seeds with red or white aril are born on the leaves or pendulous, which are very beautiful and can be used as garden trees.

Tissue culture service

Taxaceae plants are a class of trees of excellent development value. However, the slow growth rate and excessive artificial logging have significantly damaged the wild Taxaceae plant resources, and some production areas have faced extinction. The Taxaceae plant tissue culture technology service provided by Lifeasible can help to form plantations quickly and restore the ecological balance of Taxaceae plants. Tissue culture methods of Taxaceae plants mainly include in vitro embryo culture, shoot proliferation, callus regeneration, and somatic embryogenesis.

Isolated embryo culture

• We use the immature embryos from the dormant seeds of Taxaceae provided by you to cultivate them in the medium to germinate and form new plants.
• The inhibition of germination of Taxaceae isolated embryos by endogenous ABA in isolated embryos is relieved by adding gibberellin to the medium.

Shoot proliferation

• We use the shoot tips of Taxaceae provided by you as explants and culture them on MS medium to induce adventitious shoots and roots.
• The addition of low mass concentration of BA and adapted concentration of IBA to the medium facilitated the production of adventitious shoots and roots.

Callus regeneration plants

• You can choose young stems, young leaves, young shoots, and autumn stem segments as explants to induce callus. After thoroughly sterilizing the explants, we inoculated them on the culture medium. After the callus formation, shoots are further differentiated. After the shoots grow into shoots, they are transferred to a White medium containing IBA or IBA and 6-BA to determine roots and form regenerated plantlets.
• The most suitable medium for Taxaceae explant culture was MS supplemented with 0.50 mg/L 2,4-D and 0.50 mg/L NAA. B5 medium supplemented with different concentrations of NAA and 6-BA was suitable for shoot stems and leaves. 6,7-V medium containing 2,4-D, NAA, and KT were suitable for shoot explants culture.

Somatic embryogenesis

• We use the mature embryos of Taxaceae provided by you and culture them in a medium containing 2,4-D, NAA, and KT to induce callus. It is then placed on an MB medium for further culture to generate somatic embryonic cells.
• We use the Taxaceae coniferous explants provided by you to induce callus in MS medium containing 2,4-D and glutamine for cell suspension culture to produce somatic embryo cells.
• We can also use immature embryos of Taxaceae plants provided by you as explants and combine 2,4-D with BA to induce somatic embryos after 2 stages of culture.

If you are interested in our services, please feel free to contact us for more information or to discuss in detail.

You want to sign a confidentiality agreement.

You have a specific plant species for your experimental needs.

You have a reliable and relevant cooperation project to discuss.

You are very interested in our project or have any questions.

You need an updated and detailed quotation.

Contact Now