Ranunculaceae Plant Tissue Culture

Ranunculaceae plants are mostly perennial or annual herbs with a wide distribution and many species, about 50 genera, and more than 2,000 species. It is rich in a variety of chemically active ingredients, at least 30 genera of about 220 kinds of plants for medicinal purposes.

Ranunculaceae phytochemical composition is complex, with a large variety of types, the main component types are benzylisoquinoline alkaloids (BIAs) represented by magnolflorine, bisbenzylisoquinoline alkaloid, protoberberine alkaloid. There are lactones represented by triterpenoid saponins and ranunculin.

Ranunculaceae plant has analgesic and toxic effects can be used to treat bruises, rheumatoid arthritis, and other conditions may also lead to cardiac arrhythmia, etc.

Tissue culture service

The traditional propagation method of Ranunculaceae plants is by sowing or dividing, which is a long cycle, low quantity and waste of resources, so Lifeasible uses the tissue culture method for propagation of Ranunculaceae plants, which not only protects wild resources but also provides high-quality seedlings for its large-scale production and extraction, isolation and research of bioactive substances.

Some of the Ranunculaceae plants that we have successfully tissue cultured

• Ranunculus asiaticus L.
• Ranunculus natans C. A. Mey.
• Ranunculux japonicus
• Paeonia lactiflora
• Paeonia suffuticosa
• Aconitum carmichaeliiDebx.
• Aconitumvilmorinianum
• Aconitumpendulum
• Aconitum coreanum
• Aconitumkusnezoffii
• Aconitumsungpanense
• Aconitum nagarum var. lasiandrum
• Aconitumsinomontanum
• Pulsatilla chinensis (Bunge) Regel.
• Clematis florida Thunb.

Lifeasible uses the roots, stem tips and segments, leaves and petioles, anthers and receptacles, seeds, and seed embryos of Ranunculaceae plants as explants for the induction of healing tissues for regenerative cultures of Ranunculaceae plants.

Applications

• Fast breeding seedlings using tissue culture

The application of tissue culture technology for rapid asexual propagation of Ranunculaceae plants provided by Lifeasible can provide sufficient seedlings for the artificial cultivation of Ranunculaceae plants. It overcomes the disadvantages of a long growth period and slow propagation of Ranunculaceae plants.

• Production of active ingredients using tissue culture

The application of tissue culture technology of Ranunculaceae plants provided by Lifeasible for the cultivation of suspension cells, adventitious roots, and hairy roots of medicinal plants has become a very important method for the production of secondary metabolites of medicinal Ranunculaceae plants.

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