Open Tissue Culture Platform

Plant tissue culture technology is a very effective technical tool in breeding and detoxification of good seeds, production of secondary metabolites, and preservation of seed resources. However, the strict requirements for the aseptic environment in plant tissue culture technology have resulted in high investment in tissue culture production, complicated operation procedures, and serious contamination, which have restricted the application and popularity of this technology.

Figure 1. In vitro plant tissue culture: means for production of biological active compounds. (Espinosa-Leal C. A., et al., 2018)

For this reason, Lifeasible has introduced open tissue culture, a new type of tissue culture, which is an optimization of traditional tissue culture technology to improve efficiency and reduce contamination while reducing costs.

Key points and advantages of open tissue culture

Open tissue culture of plants is a way to remove plant tissue culture from a strictly aseptic operating environment without an autoclaving and ultra-clean bench and to perform plant tissue culture in a natural, open, sterile environment under the effect of inhibitors.

Open tissue culture of plants is a simplification of traditional tissue culture technology, which effectively overcomes the problems of cumbersome operation procedures, serious pollution, and high culture cost in the process of traditional tissue culture, and is one of the main directions of the development of plant tissue culture technology services provided by Lifeasible.

The advantages of the open tissue culture method provided by Lifeasible compared with traditional tissue culture

• Simplification of the operation, a significant increase in tissue culture efficiency (>2 times), and cost savings (60%).
• The open tissue culture medium is not autoclaved, with less loss of hormones and nutrients, and also the culture vessel has better light permeability. Therefore, tissue culture seedlings not only differentiate normally but also have a high differentiation rate.
• Open tissue culture seedlings grow more robustly than conventional tissue culture seedlings.

Various factors are affecting the success of open tissue culture. The core factor is to find an effective antibacterial agent that prevents contamination and has no inhibitory effect on plant growth, and aspects such as medium composition, sterilization method, and culture method also affect the success rate of tissue culture.

Therefore, Lifeasible has conducted numerous experiments on the selection of bacterial inhibitor types and concentrations, medium composition simplification, sterilization methods, and culture methods for different plant species. At present, Lifeasible has successfully carried out open tissue culture in fruits and vegetables, flowers, and medicinal plants, and other fields, and has a perfect system in determining the type and concentration of bacteriostatic agents, optimizing the composition of culture medium, selecting the sterilization method, etc., and has successfully built the open tissue culture technology system for nearly 100 species of plants to meet the different needs of customers.

Reference:

1. Espinosa-Leal C. A., et al., (2018). "In vitro plant tissue culture: means for production of biological active compounds." Planta 248, 1-18.

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