Amorphophallus Plants Tissue Culture
Amorphophallus is a perennial herb in the Araceae family with no less than 170 species, mainly distributed in some countries and regions of tropical and subtropical Asia and Africa.
The Amorphophallus plant is the only economically important crop that can synthesize konjac glucomannan in large quantities. Due to the special physicochemical properties of konjac glucomannan, the Amorphophallus plant is highly exploited in many fields such as the food industry, chemical industry, pharmaceutical industry, and agricultural environmental protection.
Tissue culture service
Amorphophallus plants are monocotyledons supported by a single petiole and usually reproduce asexually using bulb-bearing rhizomes, which has a very low reproduction coefficient and causes the accumulation of pathogenic bacteria and degradation of caste over the years, which in turn leads to a decrease in yield and deterioration in quality. Therefore, Lifeasible has established a stable breeding system of good seeds using tissue culture technology to produce Amorphophallus plant detoxification in vitro seedlings and propagate high-quality germplasm resources.
- Amorphophallus konjac
- Amorphophallus albus
- Amorphophallusrivieri
- Amorphophallus bulbifer
- Amorphophallus paeoniifolius 'Yellow'
Lifeasible has successfully established a technical system for large-scale production of Amorphophallus plant in vitro microcultures using organs such as bulb leaves, inflorescences, petioles, rhizomes, bulbs, and stem tip tissues as explants, with high annual induction rates, which can guarantee the cultivation of high-quality seedlings for large-scale production of Amorphophallus plants.
Open tissue culture
Lifeasible provides an open tissue culture method for Amorphophallus plants to address the shortcomings of the current tissue culture fast-breeding method.
Tissues of Amorphophallus plants are used as explants, inoculated into the appropriate medium, expanded to a certain number, and then removed.
The inoculated Amorphophallus plant tissues are placed in a differentiation medium, and the tissues are allowed to differentiate into a large number of shoot cells, from which the clumped shoots are differentiated and roots are grown at the same time.
- Re-differentiation culture
Amorphophallus plant tissues with differentiated clumped shoots were cultured in the re-differentiation nutrient solution to make them transform from heterotrophic to autotrophic.
Complete Amorphophallus plants are transplanted into seedbeds.
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For research use only, not intended for any clinical use.
