Theaceae Plant Tissue Culture

Theaceae Plant Tissue Culture

Camellia flowers in full bloomCamellia flowers in full bloom
Each plant has a wealth of value, not just ornamental or
medicinal, and we want to explore the possibilities of
each plant.

Theaceae is a key family in the angiosperm phylogeny, and the vast majority of Theaceae plants are evergreen shrubs or trees (some species are deciduous), widely distributed in the tropical and subtropical regions of the eastern and western hemispheres, with the greatest concentration in southern China and neighboring Southeast Asian countries. There are about 30 genera and 700 species of plants in the Theaceae, including many economic species and rare and endangered plants with high value, and many species that can be used as flowering ornamental plants.

Tissue culture service

Lifeasible has applied plant tissue culture technology to the rapid propagation of Theaceae, which is important for the conservation of germplasm resources of Theaceae species, the enrichment of garden and ornamental plant diversity, and the alleviation of the tight supply of high-quality timber and landscaping and ornamental plants.

  • Differentiation pathways in tissue culture

There are usually two pathways for cellular tissue culture to obtain regenerated plants: one is the organogenesis pathway and the other is somatic cell embryo culture. The two main differentiation pathways that we have succeeded in tissue culture of Theaceae plants are the organogenesis pathway and the somatic embryo and antherogenesis pathway.

Differentiation pathways in tissue culture - Lifeasible

  • Basic elements of tissue culture
  • Selection of explants

The selection of explants is one of the key factors for the success of plant tissue culture. The age, developmental stage, growth environment, and location of the explant donor plants can lead to differences in the physiological status of the explants, thus affecting the morphogenesis of tissue culture. We usually select stem tips, stem segments, seeds, buds, anthers, etc. as explant materials for culture.
Note: Please back up your experimental materials beforehand, and contact our staff immediately for an accurate response if you have any special needs.

  • Commonly used culture media

A large number of elements in the basic medium have a great influence on the tissue culture of Theaceae plants. Our basic medium for the tissue culture of Theaceae plants is mainly MS, 1/2 MS, modified MS, ER, MT, etc.

  • Frequently used exogenous hormones

Plant growth regulating substances are key substances in the culture medium and play an important regulatory role in tissue culture, although the amount is very small. The type of hormone and the ratio of different functional hormones are one of the key factors affecting the successful initiation of explants. The hormones we often use in the culture of Theaceae plants are 2, 4-D, 6-BA, NAA, etc. Nutrients such as coconut milk and hydrolyzed milk protein are also used by us in the tissue culture of Theaceae plants.

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