Elaeagnaceae Plants Tissue Culture

Elaeagnaceae plants have about 50 species in 3 genera and are mainly distributed in temperate and subtropical regions of the Northern Hemisphere. There are both trees and shrubs in this family, and most species are drought-tolerant, but some are tolerant of humid climates. Among them, Hippophae rhamnoidese L. is an excellent tree species for wind and sand control, soil and water conservation, and environmental improvement, etc. It is also widely used in medical, food, and health care, and is an important economic tree species. However, most of the tree species are still in the wild and unexplored, and a large number of wild tree species with ornamental and high application value for gardening have not yet attracted sufficient attention, and tissue culture is a very effective means.

Tissue culture service

Elaeagnaceae plants have high ecological and economic values, and with the accelerated construction of Elaeagnaceae plant resources and the sharp increase in the demand for good seeds, the rapid propagation of Elaeagnaceae plants, especially Elaeagnaceae plant tissue culture technology, has become a very important tool. Lifeasible has optimized four aspects of Elaeagnaceae plants, namely, explant culture, multiplication culture, rooting culture, and domestication transplantation, and selected more suitable explant types and culture media, multiplication media, rooting media, and domestication transplantation technology systems, which can meet the needs of large-scale rapid propagation of Elaeagnaceae plants and plant nursery.

• Elaeagnus angustifolia L.
• Elaeagnus umbellate Thunb.
• Elaeagnus conferta
• Elaeagnus mollis
• Hippophae rhamnoides L.

Key points of tissue culture

• The first thing to address in the tissue culture of Elaeagnaceae plants is the selection of suitable explants. Lifeasible has been able to induce regenerated seedlings in tissue culture of Elaeagnaceae plants using dormant stem segments, axillary buds of dormant branches sprouted by hydroponics, young branches, young leaves, and seeds of adult plants as explants.
• Among them, hydroponically grown stem tip is currently one of the more selected explants in the tissue culture of Elaeagnaceae plants, which has the advantages of young material, easy sterilization, less contamination, wide adaptation to basic medium and hormones, rapid growth after inoculation, and easy establishment of sterile lines.
• The main method suitable for Elaeagnaceae plant explants culture is solid culture, Lifeasible mainly adopts the basic medium such as MS, B5, WPM, etc., among which MS-1/4 MS is the most used in the basic medium, and the main hormones used are 6-BA, IBA, IAA, NAA and KT.

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