Dioscoreaceae Plant Tissue Culture

Dioscoreaceae family belongs to the angiosperms monocotyledonous phylum Liliaceae (system), a small family in the plant classification, with 9 genera and 650 species, widely distributed in tropical, subtropical, and warm temperate regions of the world, especially the most species in tropical America, intermittently distributed around the world.

Although the Dioscoreaceae family is small in plant classification, the plants of this family are not included in the classification. However, synthetic hormones can be extracted from the plants of this family.

Dioscin is widely found in Dioscoreaceae plants, are natural steroidal saponins, Dioscoreaceae plants are mostly herbaceous twining vines, with tuberous or root-like woody underground stems, and dioscin in Dioscoreaceae plant rhizomes high content, with expectorant, anorexia, diuretic, soothe the tendons and activate blood, etc. It has various pharmacological effects such as improving cardiovascular function, protecting the liver, regulating body immunity, anti-platelet aggregation, lowering blood lipids, and anti-cancer.

Tissue culture service

Since the tubers of most medicinal species are slow-growing, irregularly shaped and unfavorable for harvesting, and susceptible to adverse environment and pests and diseases, the current researchers' objectives for improving Dioscoreaceae plants are mainly focused on making them shorter in the growth period, stable and high-yielding in traits, resistant to diseases, and regular in tuber shape. However, traditional breeding and conventional cultivation techniques are difficult to meet the needs, which to some extent limit the research of Dioscoreaceae plants.

Therefore, the use of the tissue culture method by Lifeasible for the improvement of Dioscoreaceae plant varieties and the selection of new varieties will be a more feasible and effective way at present. This depends, first of all, on the high quality of healing tissues obtained by Lifeasible through tissue culture techniques, and the regeneration system of plants after gene transformation. And we have established stable tissue culture systems in several species of this family.

• Dioscorea zingiberensis
• Dioscorea opposita Thunb.
• Dicorea composita
• Dioscorea bulbifera L.
• Dioscorea deltoidea
• Dioscorea collettii
• Dioscorea nipponica Makino

In the tissue culture of Dioscoreaceae plants, Lifeasible constructed tissue culture systems using rhizomes, young stem cuttings, petioles, and leaves as explants. We performed tissue culture on LS, W, M9, B5, MS, and modified MS media, which showed high induction rates and their induction yielded healing tissues that were less susceptible to aging and better able to produce metabolites.

• Direct induction of adventitious shoots pathway
• Healing tissue differentiation adventitious shoot pathway
• Microtuber propagation pathway

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