Rare plants usually refer to plants that are on the verge of extinction due to natural factors or man-made destruction. On the one hand, rare and endangered plants are characterized by low fruiting rates, large inter-annual fluctuations, and obvious small and large years in their natural environment. Combined with the characteristics of animals feeding on immature and surface seeds, as well as the easy decay of seeds buried in the ground, the natural reproductive capacity of seeds of rare tree species is extremely weak, and the number of populations is decreasing.
On the other hand, with the increasing demand for wild plant resources, habitat destruction, predatory exploitation, climate change, and other factors have led to a continuous decline in wild plant resources, towards endangerment and loss of plant diversity.
The genetic traits of live seedlings are unstable, and there are many drawbacks such as the unevenness and low productivity of thriving trees, so the use of live seedlings to raise seedlings is far from meeting the market demand. Lifeasible uses tissue culture to fast-track the propagation of rare plants, not only to overcome the many problems in seed propagation but also to overcome the disadvantages of the slow and low reproduction rate of conventional asexual propagation.
Because tissue culture has the advantages of being independent of seasonal and climatic constraints, land-saving, fast, high quality, technology-intensive, and easy for intensive management and factory production, our application of plant tissue culture technology to the rapid propagation of rare plants has important practical applications to protect the germplasm resources of rare plants, enrich plant diversity, and alleviate the tension of rare plant resources.
Some of the rare plants that we have successfully tissue cultured
Species | Explants | Differentiation Pathway |
Almphda spinulosa | Spores | Spore propagation, healing tissue |
Davidia involucrata | Winter buds | Buds |
Camellia chrysantha | Cotyledons, stem tip, single bud | Indeterminate buds, pseudo-bead buds, buds |
Panax ginseng | Leaf blade, petiole, corolla stalk, root, anther | Healing tissue, somatic embryo, protoplasts |
Platycerium wallichii | Young sporophyte | GGB protoplasts |
Cephalotaxus mannii | Young stems, young leaves | Healing tissues |
Cinkgo biloba | Stem segments, stem tips, mature embryo, young leaves | Buds, healing tissue, germination of embryos |
Pseudotaxus chienii | Young stems, young leaves | Healing tissues |
Heplacrclium miconioides | Winter buds, young leaves, flowers | Healing tissue |
Gymnocarpos przewalskii | Hypocotyl | Healing tissue |
Psammosilene tunicoides | Stem tips, stem segments with shoots | Buds |
Helianthemum soongoricum | Cotyledons, stem, leaf | Healing tissue, somatic cell embryo |
Elaeagnus mollis | Stem, cotyledon | Healing tissue |
Eucommia ulmoides | Cotyledons, embryonic axis | Healing tissue |
Oryza granulata | Seeds | Healing tissue, protoplasm |
Oryza rufipogon | Seeds | Healing tissue |
Juglans regia | Germinated stem segments | Buds |
Manglietia patungensis | Winter buds | Buds |
Eurycorymbus cavaleriei | Terminal bud, hypocotyl | Buds, healing tissue |
Litchi chinensis Sonn. var. euspontanea | Seeds | Parenchyma |
Camellia reticulata | Stem tip | Buds |
Tetraena mongolica | Cotyledons, embryonic axes, roots | Healing tissue, somatic cell embryo |
Emmenopterys henryi | Bud, leaf | Buds, healing tissues |
* The list of species that can be tissue cultured is constantly being updated, so please stay tuned.
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