Paphiopedilum Plant Tissue Culture
Paphiopedilum has a high ornamental value due to its unique flower shape, brilliant flower color, and long-lasting flowering period, and is a very popular high-end flower in the international flower market.
Paphiopedilum orchid flowers
Our stable tissue culture system is the golden
finger to win the trust of our customers
However, Paphiopedilum is far from meeting the market demand due to the difficulty of seedling propagation and long cultivation period, which leads to the over-harvesting of its wild resources.
The traditional propagation method of Paphiopedilum is often by dividing the clumped pseudobulbs and cultivating them independently, but the reproduction coefficient is low and the reproduction rate is slow, which makes it difficult to meet the needs of species conservation and commercial production. To effectively conserve and sustainably utilize Paphiopedilum germplasm resources, it is crucial to break through the bottleneck of its propagation technology.
Factors affecting the non-symbiotic germination of Paphiopedilum seeds include seed maturity, pretreatment methods, medium composition (including basic medium, pH value, carbon source, plant growth regulators, organic additives, activated carbon), culture methods (solid culture, liquid culture, and liquid-solid layered culture, etc.) and culture conditions (including culture temperature and light).
Symbiotic fungi play a key role in the seed germination of Paphiopedilum plants and are specific.
Tissue culture service
CD BioScience uses tissue culture for large-scale clonal propagation to reduce the collection pressure on wild Paphiopedilum plants and to meet the demand for commercial seedling production.
- Selection of explants (related to the target material)
Based on our extensive experience, we recommend that you preferably save Stem tip, leaf, seed, pedicel, pedicel axillary bud, lateral bud, flower bud, stem segment, root tip of plants and provide them to our staff as explants for culture experiments.
Note: Please back up your experimental materials beforehand, and contact our staff immediately for an accurate response if you have any special needs.
- Plant regeneration method
Healing tissue or protoplast-like induction regeneration pathway
Clonal shoot regeneration pathway
- Commonly used culture media
Heller, MS, 1/2MS, modified MS, etc.
- Frequently used exogenous hormones
2,4-D, NAA, BA, ZT, KT, etc.
Sucrose, maltose, coconut milk, hydrolyzed casein, etc.
You want to sign a confidentiality agreement.
You have a specific plant species for your experimental needs.
You have a reliable and relevant cooperation project to discuss.
You are very interested in our project or have any questions.
You need an updated and detailed quotation.
For research use only, not intended for any clinical use.
