Ploidy and Trait Analysis Service for Tissue Culture Plants

Plant chromosome ploidy is a common phenomenon in nature. Haploids and their doubled diploid lines are genetically identical and are essential in plant cytology, crop genetics, and breeding. Lifeasible can be used to segregate ploidy and traits in tetraploid and hexaploid hybrid regenerated plants through tissue culture to obtain stable lines in both ploidy and traits.

What is Plant Ploidy

Chromosome ploidy plays a significant role in plant evolution and crop variety improvement and will play an increasingly important role in genetic breeding and the utilization of excellent agronomic traits. Ploidy identification is an essential part of ploidy breeding and its application; how to apply the most simple, effective, and as early possible to identify the ploidy level of the plant can significantly reduce the workload of breeding and blindness, reduce costs, accelerate the breeding process, in crop ploidy breeding is of great significance.

Figure 1. Fluorescence in situ hybridization (FISH) to identify genomes in polyploids. (Heslop-Harrison, J. S. P., et al, 2023)

After plant ploidy, its genetic behavior is more complex. In the process of ploidy breeding and its application, it is necessary to test the purity of seeds to understand the genetic background and genetic effects of mixed populations. A combination of identification methods can be used to determine the accuracy of the identification.

What We Offer

We obtained regenerated plants using tissue culture techniques and identified their ploidy by morphological identification, physiological and biochemical indicators, fertility status, cytological identification, and chromosome counting. Finally, through propagation, we identify the ploidy of the regenerated plants and the stability of the traits. It helps you to accelerate the breeding process, shorten the breeding cycle, and improve the efficiency of selection and breeding.

• Differences in plant ploidy cause changes in physiological characteristics, leading to corresponding changes in physiological and biochemical indicators. With the help of this characteristic, we can help you identify plant ploidy by measuring water holding capacity, osmotic pressure, respiration, sugar content, vitamin content, mineral content, amino acid content, photosynthetic rate, transpiration rate, isoenzymes, changes in enzyme bands and so on.
• Flow cytometry is a modern analytical technique with the advantages of simple sample preparation, low dosage, efficient and fast operation, and high data reproducibility. This allows us to provide you with fast and large quantities of chromosome ploidy analysis services.

Advantages

• Isolation and purification of hybrids using tissue culture to create stable plants and germplasm resources.
• Accurately determine plant ploidy by using a combination of identification methods.
• We are experienced in meeting your different needs.

Accurate identification of plant ploidy level is an important part of its function, and Lifeasible uses tissue culture and a variety of methods to achieve rapid, time-saving, and labor-saving identification of plant ploidy and to obtain strains that are stable in both ploidy and traits, to serve the use of ploidy plants better. Please feel free to contact us for customized solutions.

Reference

1. Heslop-Harrison, J. S. P., et al. (2023). "Polyploidy: its consequences and enabling role in plant diversification and evolution." Ann Bot 131(1):1-10.

You want to sign a confidentiality agreement.

You have a specific plant species for your experimental needs.

You have a reliable and relevant cooperation project to discuss.

You are very interested in our project or have any questions.

You need an updated and detailed quotation.

Contact Now