Caricaceae Plants Tissue Culture

Caricaceae are small trees, shrubs, or herbs, with six genera (Carica, Cylicomorpha, Horovitziana, Jacaratia, Jarilla, Vasconcellea) and about 47 species, native to the tropics of western Africa and America, and have been introduced in tropical and subtropical regions around the world.

In particular, Carica papaya L. belongs to Carica, a tropical and subtropical evergreen soft woody large perennial herb native to southern Mexico and adjacent Central America, whose fruits are rich in nutrients and both fruits and seeds can be used as medicine with many health benefits.

Tissue culture service

Currently, Caricaceae plants are mainly produced as seedlings. The complexity of papaya strains not only results in high variability in the offspring but also requires the removal of male and female plants at flowering and the retention of hermaphrodite plants, which makes the production cost high and is not conducive to the industrial development of Caricaceae plants. Lifeasible is an effective solution to this problem by using tissue culture asexual propagation technology to grow seedlings of Caricaceae plants.

Compared with seed seedlings, Lifeasible' tissue-cultured Caricaceae seedlings have the characteristics of fast growth and vigorous growth in the field and can eliminate virus plants from the source. It can generally flower and fruit earlier, which not only saves planting cost, but also the tissue-bred seedlings bred from hermaphroditic plants of Caricaceae plants can increase the proportion of oblong fruits, which improves the economic benefits of Caricaceae plants while reducing the cost of seedlings, and has good product competitiveness.

Key points of tissue culture

Importantly, Lifeasible has overcome many problems in tissue culture of Caricaceae plants, including low success rate of explant induction, insufficient number of effective shoots in succession culture, low quality of root system, and unstable rooting induction rate.

• Optimizing the exosome collection process

Lifeasible strictly controls the growth environment of mother plants of Caricaceae in the process of culturing them, such as carrying out greenhouse culture to ensure good lateral bud emergence, improve the quality of collected lateral buds, and also facilitate the cutting of sprouting strips.

• Optimize the way of disinfection of explants

Lifeasible adopts a combination of disinfection methods to ensure that the surface of the explants is completely disinfected by killing the bacteria and ensuring the surface disinfection of Caricaceae plant explants is complete.

• Sterile shoot culture to increase the effective shoot production rate

Lifeasible performs secondary robust bud culture for adventitious buds that do not meet the criteria for effective buds. Alternating the two mediums of succession culture can effectively improve the proliferation coefficient of adventitious shoots and promote the normal turnover of successive seedlings while also simplifying the culture steps and reducing the production time.

• Increasing effective rooting rate with two-step rooting method

Lifeasible has adopted a two-step rooting method for rooting induction to effectively increase the rooting rate of germlings, which can promote the production of effective roots with clear root systems and improve the survival rate of tissue culture seedlings.

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