Bignoniaceae Plant Tissue Culture
Pyrostegia venusta with the orange petals belongs to
the Bignoniaceae family.
Bignoniaceae has excellent ornamental value, and we
provide plant tissue culture technology services to
meet the market demand for Bignoniaceae plant
seedlings.
Bignoniaceae belongs to the class Dicotyledoneae and the order Tuberose, with 110 genera and about 650 species, which are widely distributed in tropical and subtropical regions. Bignoniaceae have variable leaf shapes and left-right symmetrical flowers that form terminal, axillary cymes, panicles, or racemes, which are usually large and beautiful.
Some Bignoniaceae plants are used as medicine for various ailments, such as childhood convulsions, fever, and malaria. Most of the flowers of this family are large, beautiful, and brightly colored, making them an excellent choice for ornamental trees in gardens. The trees of Bignoniaceae, with hardwood, are also ideal for timber and reforestation.
Tissue culture service
Bignoniaceae has various economic values, such as ornamental, medicinal, and wood. However, the traditional methods of sowing, grafting, and cutting have been challenging to meet the development needs of the modern Bignoniaceae plant industry.
Lifeasible has conducted long-term research in plant tissue culture and accumulated rich experience. Our plant tissue culture scientists can quickly realize large-scale tissue culture of Bignoniaceae for customers based on advanced plant culture platforms.
We recommend you provide shoots of ungerminated annual Bignoniaceae plants or young stems with leaves grown after hydroponics as explants.
- Selection of culture medium
The choice of medium greatly influences the induction of differentiation in Bignoniaceae plants. MS, DKW, WPM, 1/2MS, B5, and N6 are all commonly used media for tissue culture of Bignoniaceae plants.
However, combined with years of experience in tissue culture, we found that MS is suitable for stem segment culture and can help induce axillary buds; DKW can promote the induction of new buds with moderate internodes; B5 culture has earlier sprouting of explants with faster growth and elongated leaves. N6 promotes the production of seedlings with long internodes, thick stems, and small leaves.
Under aseptic conditions, the induced axillary bud tips were cut and immediately inoculated in an N6 medium with appropriate BA and NAA to promote the proliferation culture of the buds.
- Rooting culture and transplanting
When the adventitious buds inoculated on the induction medium grow small leaves, the upper stem tip is cut and transferred to the rooting medium for rooting culture. The seedlings can be transplanted to the greenhouse when they have produced a proper number of strong roots and are about 3-4 cm tall.
The preparation of hormone ratios and asepsis are critical points for successful tissue culture of Bignoniaceae plants. Lifeasible has conducted several experiments to determine the ratio of NAA, IBA, and other hormones required for different Bignoniaceae plants. We guarantee to provide you with a high survival rate and seedling emergence rate.
We have a strict aseptic system where plant tissues are sterilized for a reasonable length and number of times to ensure a reduced contamination rate without affecting tissue activity.
If you are interested in our services, please feel free to contact us for more information or to discuss in detail.
You want to sign a confidentiality agreement.
You have a specific plant species for your experimental needs.
You have a reliable and relevant cooperation project to discuss.
You are very interested in our project or have any questions.
You need an updated and detailed quotation.
For research use only, not intended for any clinical use.
