The firm leaves of the Ilex
plant and the red berries dotted with them
Because traditional methods always have insurmountable
drawbacks, the tissue culture technology services we offer
are very important for the propagation of Ilex plants.
The Ilex plant is the only genus in the family Aquifoliaceae with about 400 species. Ilex plant is a flowering plant with a height of about 2 to 25 m. Some are trees and some are shrubs.
Ilex plants mostly prefer warm and humid environments but are highly adaptable, so they are widely distributed in Asia, Europe, northern Africa, North, and South America, and are typical of subtropical zonal vegetation.
The leaves of Ilex plants are firm and glossy, and the berries are bright red and clustered on the branches. So, the ornamental trees of Ilex plants have high ornamental value because of their beautiful tree posture, peculiar leaf shape, beautiful flowers, and other garden morphological characteristics, in addition to the fruits. And now a variety of Ilex plants have become very common ornamental trees in the garden.
Tissue culture service
Ilex plants have great value and are widely used in roads, parks, gardens, industrial and mining areas, etc. However, it seems that not many species of Ilex plants are used today. The main reason is that the seed germination rate of Ilex plants is low and the survival of cuttings is difficult. Therefore, Lifeasible has established an efficient tissue culture propagation technology platform, which has greatly promoted the application of Ilex plants in gardens and other fields, solved the problems of low reproduction coefficient, slow reproduction speed, and easy degradation of quality, and better realized the economic value of Ilex plants.
The selection of explants is also a key step in plant tissue culture. We have applied tissue culture techniques to the cultivation and propagation of holly plants, starting with the initial use of ex vivo culture of young embryos, and now we also obtain regenerated plants by using stem segments, stem tips, leaves, young shoots, petals, pollen, etc. as explants for culture.
- Culture media and plant additives
For the tissue culture of woody plants, the selection of a suitable basic medium is of great importance for the induction of differentiation of explants and organ regeneration. Different basic media differ in the composition and content of essential nutrients. We mainly use three basic media, MS, B5, and WPM, in the tissue culture of Ilex plants.
Plant growth regulators play an important regulatory role in plant growth and development; therefore, the addition of different concentrations and ratios of plant growth regulators to plant culture media can lead to a wide variation in exosome induction and differentiation. The plant growth regulators we commonly use in Ilex plants are 6-BA, NAA, IBA, GA3, ABT, AC, etc.
Using the tissue culture technology we provide, hundreds of thousands of Ilex plant tissue culture seedlings (bottle seedlings) can be grown in a short period, greatly improving the propagation efficiency of the genus. However, we have developed a complete system to completely overcome these problems.
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