Size
48 T
Description
PCR-LFD technology was used to amplify the target gene in vitro in combination with specific amplification primers. The PCR amplification products are detected using lateral flow chromatography test strip (LFD) technology, and based on the results of the disposable nucleic acid colloidal gold test strip test line (T), it is determined whether the sample to be examined contains the target gene or not, and the qualitative analysis of the test results is realized. This kit has the advantages of high sensitivity, high specificity, short reaction time, easy operation, and low price.
Reaction Volumes
25 μL
Reagent Type
Fluid
Advantages
Good specificity, amplified by PCR technology.
High sensitivity, the detection sensitivity can reach below 1,000 copies/μL.
Easy to operate, using one-step PCR for amplification, reverse transcription step, and PCR amplification are completed in one tube of reaction solution.
Protocol
See the instruction manual for details.
1. Sample processing (sample processing area). Sample pre-treatment -> DNA extraction.
2. Reagent preparation (reagent preparation area).
3. Sample addition (sample processing area).
4. PCR amplification (nucleic acid amplification area).
5. Result analysis and determination.
Tips
1. Sample test results are related to the quality of sample collection, processing, transportation, and preservation.
2. False positive results can occur when cross-contamination is not controlled during sample extraction.
3. Leakage of positive controls and amplification products can lead to false positive results.
4. Genetic mutation and recombination of pathogens during the epidemic process will lead to false negative results.
5. Differences in extraction efficiency between different extraction methods can lead to false negative results.
6. False-negative or quantitatively inaccurate results may occur due to decreased detection efficiency of reagents caused by improper transportation, storage, or inaccurate preparation of reagents.
Precautions
1. All operations should be carried out in strict accordance with the instructions.
2. The components in the kit should be naturally melted, completely mixed, and briefly centrifuged before use.
3. The reaction solution should be kept away from light.
4. Avoid the presence of air bubbles during the reaction, and the tube should be tightly capped.
5. Sample processing, reagent preparation, and spiking should be done in different areas to avoid cross-contamination.
6. All items in the kit should be treated as contaminants.
Applicable Instruments
Gradient PCR instrument.
Required: 1.5 mL centrifuge tubes without DNA/RNA enzymes, 0.2 mL PCR reaction tubes, cartridge tips (10 μL, 200 μL, 1,000 μL), centrifuge, oscillating mixer, and pipettes of various sizes.
Storage and Validity Period
-20℃±5℃. Keep away from light, transportation, repeated freezing, and thawing less than 7 times. Valid for 12 months.
